We developed a method to measure dissolved inorganic carbon and phosphate uptake in the same sample:

 

Duhamel S., Zeman F., and T. Moutin, 2006. A dual-labeling method for the simultaneous measurement of dissolved inorganic carbon and phosphate uptake by marine planktonic species. Limnology and Oceanography: Methods 4, 416-425. Open access

 

We successfully applied this method in the southeast subtropical Pacific ocean during the BIOSOPE cruise:

 

Duhamel S., and T. Moutin, 2008. C and P incorporation rates of microbial assemblages in contrasting environments in the Southeast Pacific. Marine Ecology Progress Series 375, 53-64. DOI: 10.3354/meps07765MEPS. Open access

 

We also evaluated the use of a phosphorus based proxy to measure microbial growth rates:

 

Duhamel S., Moutin T., Van Mooy B., Van Wambeke F., and H. Claustre, 2007. Growth and specific P-uptake rates of bacterial and phytoplanktonic communities in the Southeast Pacific (BIOSOPE cruise). Biogeosciences 4, 941-956. Open access

 

These results were in good agreement with phospholipids synthesis rates:

 

Van Mooy B., Moutin T., Duhamel S., and F. Van wambeke, 2008. Phospholipids synthesis rates in the eastern subtropical South Pacific Ocean. Biogeosciences 5, 133-139. Open access

 

We evaluated the role of phosphate availability in controling nitrogen fixation in the North, Southwest and Southeast Pacific ocean:



Moutin T., Karl D.M., Duhamel S., Rimmelin P., Raimbault P., Van Mooy B., and H. Claustre, 2008. Phosphate availability and the ultimate control of new nitrogen input by nitrogen fixation in the tropical Pacific Ocean. Biogeosciences 5, 95-109. Open access



 

Finally, we described longitudinal and temporal trends in heterotrophic prokaryotic production and its coupling with primary production:

 

Van Wambeke F., Obernosterer I., Moutin T., Duhamel S., Ulloa O. and H. Claustre. 2008. Heterotrophic prokaryotic production in the South East Pacific: longitudinal trends and coupling with primary production.  Biogeosciences 5, 157-169. Open access



Van Wambeke F., Tedetti M., Duhamel S., and R. Sempéré, 2009. Diel variability of heterotrophic bacterial production and underwater UV doses in the eastern South Pacific. Marine Ecology Progress Series 387, 97–108. DOI: 10.3354/meps08075. Access